polyclonal rabbit anti erp57  (Novus Biologicals)

Journal: Viruses

Article Title: PDIA4 Is a Host Factor Important for Lymphocytic Choriomeningitis Virus Infection.

doi: 10.3390/v15122343

Figure Lengend Snippet: Figure 2. The PDIA4 expression level is upregulated by LCMV infection both in vitro and in vivo. (A) The expression level of LCMV-NP in A549 cells at 12, 24, 36, and 48 h.p.i. determined by RT-PCR. (B) The expression level of detectable PDIs in A549 cells at 12, 24, 36, and 48 h.p.i. determined by RT-PCR. (C) The protein expression level of PDIA3, PDIA4, PDIA5, and PDIA6 in mock-/LCMV- infected A549 cells at 12, 24, 36, and 48 h.p.i. determined by Western blot. (D) The protein expression level of PDIA3, PDIA4, PDIA5, and PDIA6 in mock-/LCMV-infected HEK293T cells at 12, 24, 36, and 48 h.p.i. determined by Western blot. (E) The protein expression level of PDIA3, PDIA4, PDIA5, and PDIA6 in mock-/LCMV-infected NHBE cells at 48 h.p.i. determined by Western blot. (F) The relative mRNA expression level of PDIA4 in the kidney and lung of mock-/LCMV-infected mice (relative to mock, n = 5, unpaired t test). *** p < 0.001, **** p < 0.0001.

Article Snippet: The anti-PDIA3 rabbit polyclonal antibody (15967-1-AP), antiPDIA4 rabbit polyclonal antibody (14712-1-AP), anti-PDIA5 rabbit polyclonal antibody (15545-1-AP), anti-V5 antibody (14440-1-AP), horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG(H+L) (SA00001-2), and HRP-conjugated goat anti-mouse IgG(H+L) (SA00001-1) were purchased from Proteintech Group (Wuhan, China).

Techniques: Expressing, Infection, In Vitro, In Vivo, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: iScience

Article Title: A KO mouse model for the lncRNA Lhx1os produces motor neuron alterations and locomotor impairment

doi: 10.1016/j.isci.2022.105891

Figure Lengend Snippet: Lhx1os protein interactors analysis (A) qRT-PCR analysis of Lhx1os enrichment in PDIA3-immunoprecipitate expressed as fold change compared with IgG-immunoprecipitate. Data presented as the mean ± SD of three independent experiments. Statistical analyses were performed using Student t -test (∗p < 0.05). (B) Co-staining of Lhx1os RNA (red) and HB9-GFP (green) in mESC-derived MNs untreated (-Tg) or treated (+Tg) for 2 h with the ER stress inducer thapsigargin (Tg). DAPI, 4′,6-diamidino-2-phenylindole. HB9, motor neuron and pancreas homeobox 1. Scale bar = 10 μm. (C) Quantification of the mean number of spots per MN in cells untreated (-Tg; n = 66) or treated (+Tg; n = 283) with Tg. Data are presented as the mean ± SEM. Statistical analyses were performed using Student t -test (∗∗∗p < 0.001). (D) qRT-PCR analysis of Lhx1os RNA in mESC-derived MNs exposed to Tg (300 nM) and collected after 4 h of treatment (+Tg) compared to untreated cells (-Tg). The RNA expression levels were normalized against GAPDH mRNA and expressed as relative quantities with respect to untreated cells set to a value of 1. Data are presented as the mean ± SD of three independent experiments. Statistical analyses were performed using Student t -test (∗∗p < 0.01). (E) Representative SIM caption performed on mESCs-derived MNs 3 days after dissociation: Basescope™ FISH for Lhx1os (red) and immunofluorescence for PDIA3 (gray) and KDEL (green). The selected region of interest (white square) was enlarged inside the micrographic image. Nucleus was marked with DAPI (blue signal). Scale bar corresponding to 5 μm. (F) Digital magnification and volume view of region depicted in (E). Pearson’s correlation coefficients (PCC) that indicates co-localization index between Lhx1os and PDIA3 or KDEL fluorescence is shown. (G) Fluorescence intensity (FI) profile of Lhx1os, PDIA3, and KDEL signals, obtained by line-scan method on the region of interest showed in the insert. Fluorescence scanning of single SIM channels is color-coded. Red arrow points the strong overlapping of Lhx1os/PDIA3 fluorescence signals with respect to KDEL profile. The black line in the insert indicates the row of pixel where the intensity values are measured.

Article Snippet: Rabbit polyclonal anti-ERp57 (PDIA3) , Abcam , cat#ab10287; RRID: AB_297026.

Techniques: Quantitative RT-PCR, Staining, Derivative Assay, RNA Expression, Immunofluorescence, Fluorescence

Journal: iScience

Article Title: A KO mouse model for the lncRNA Lhx1os produces motor neuron alterations and locomotor impairment

doi: 10.1016/j.isci.2022.105891

Figure Lengend Snippet: Effects of Lhx1os and Pdia3 in vitro depletion in N2a cells (A) qRT-PCR analysis of the indicated RNAs in N2a cells exposed to Tg (500 nM) and collected after 2 h of treatment (+Tg) compared to untreated cells (-Tg). The RNA expression levels were normalized against GAPDH mRNA and expressed as relative quantities with respect to untreated cells set to a value of 1. Data are presented as the mean ± SD of 3 independent experiments. Statistical analyses were performed using Student t -test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001). (B) qRT-PCR analysis of the indicated RNAs in N2a cells treated with scramble siRNAs (siScr) and siRNAs against Lhx1os (siLhx1os) or Pdia3 (siPdia3) and exposed (+Tg) or not (-Tg) to thapsigargin (500 mM) for 2 h. The RNA expression levels were normalized against GAPDH mRNA and expressed as relative quantities with respect to siScr-treated cells set to a value of 1. Data are presented as the mean ± SD of at least 3 independent experiments. Statistical analyses were performed using Student t -test (ns p>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).

Article Snippet: Rabbit polyclonal anti-ERp57 (PDIA3) , Abcam , cat#ab10287; RRID: AB_297026.

Techniques: In Vitro, Quantitative RT-PCR, RNA Expression

Journal: iScience

Article Title: A KO mouse model for the lncRNA Lhx1os produces motor neuron alterations and locomotor impairment

doi: 10.1016/j.isci.2022.105891

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-ERp57 (PDIA3) , Abcam , cat#ab10287; RRID: AB_297026.

Techniques: Virus, Subcloning, Recombinant, Saline, Modification, Sterility, Knock-Out, Transfection, Protease Inhibitor, Membrane, Staining, Electron Microscopy, Plasmid Preparation, DNA Extraction, RNAscope, Clone Assay, Irradiation, Negative Control, Expressing, Software, Microscopy

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 1. The mRNA expression of PDIA3 in multiple human cancers. (A) The mRNA level of PDIA3 in different kinds of cancer lines, which were obtained from TIMER database. (B-S) The mRNA expression of PDIA3 in several malignancies, which were based on TCGA and GTEx normal tissues obtained from GEPIA datasets. *P value <0.05, **P value <0.01, ***P value <0.001.

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques: Expressing

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 2. The overall survival (OS) analysis of the expression of PDIA3 in various cancers was performed by using the GEPIA database. The OS plot of PDIA3 in BLCA (A), BRCA (B), CESC (C), CHOL (D), COAD (E), ESCA (F), HNSC(G), KICH (H), KIRC (I), KIRP (J), LIHC (K), LUAD (L), LUSC (M), PRAD (N), READ (O), STAD (P), THCA (Q), UCEC (R).

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques: Expressing

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 3. The disease-free survival (DFS) analysis of the expression of PDIA3 in various cancers was performed by using GEPIA database. The RFS plot of PDIA3 in BLCA (A), BRCA (B), CESC (C), CHOL (D), COAD (E), ESCA (F), HNSC(G), KICH (H), KIRC (I), KIRP (J), LIHC (K), LUAD (L), LUSC (M), PRAD (N), READ (O), STAD (P), THCA (Q), UCEC (R).

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques: Expressing

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 4. The mRNA and the protein expression of PDIA3 in LUAD. (A) The mRNA level of PDIA3 in multiple cancers was determined by using Oncomine database. (B) The mRNA expression of PDIA3 was investigated by R software. (C) The mRNA and the protein level of PDIA3 were validated by qRT-PCR. (D, E) The protein level of PDIA3 were validated by IHC and western blotting analysis analysis respectively.

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques: Expressing, Software, Quantitative RT-PCR, Western Blot

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 5. The relationship between the expression of PDIA3 and the clinicopathological parameters were determined by UALCAN database. The relationship between PDIA3 and the patient’s age (A), gender (B), race (C), smoking habits (D), cancer stages (E), and nodal metastasis status (F) was analyzed, respectively.* P value <0.05, **P value <0.01, *** P value <0.001.

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques: Expressing

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 6. GO enrichment analysis of PDIA3 in LUAD was determined by performing Spearrman’s correlation test. (A) BP, biological process. (B) CC, cellular component. (C) MF, molecular function.

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques:

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 7. miR-301a-5p was identified as a potential upstream miRNA of PDIA3 in LUAD. (A) The miRNA-PDIA3 regulatory network was established by Cytoscape software. (B) The expression correlation between miRNAs and PDIA3 was predicted in LUAD by using starBase database. (C) The expression of miR-301a-5p in LUAD was compared to that in adjacent normal tissues and analyzed by starBase database. (D) The prognostic value of miR-301a-5p in LUAD was assessed by Kaplan – Meier plotter. (E) qRT-PCR was used to detect PDIA3 levels in LUAD tissues (N = 18). (F) qRT-PCR was used to detect miR-301a-5p levels in LUAD tissues (N = 18). (G) Pearson analysis was used to evaluate correlation of PDIA3 and miR-301a-5p mRNA levels in LUAD tissues (N = 18).

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques: Software, Expressing, Quantitative RT-PCR

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 8. The correlation between immune cell infiltration and PDIA3 expression in LUAD. (A) The infiltration level of various immune cells was estimated under different copy numbers of PDIA3 in LUAD. (B-G) The relationship of PDIA3 level and B cell (B), CD8+ T cell (C), CD4+ T cell (D), macrophage (E), neutrophil (F), and dendritic cell (G) infiltration level in LUAD.

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques: Expressing

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 10. The model of miR-301a-5p/ PDIA3 axis during the progression of LUAD.

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques:

Journal: Genomics

Article Title: MicroRNA-mediated high expression of PDIA3 was correlated with poor prognosis of patients with LUAD.

doi: 10.1016/j.ygeno.2022.110417

Figure Lengend Snippet: Fig. 9. The correlation of PDIA3 expression with PD- 1, PD-L1, and CTLA-4 expression in LUAD.(A) Spearman correlation of PDIA3 was based on purity and obtained by using TIMER database. (B) Spearman correlation of PDIA3 indicated the expression of PD- L1 in LUAD, and it was adjusted according to purity by using TIMER database. (C) Spearman correlation of PDIA3 elicited the expression of CTLA-4 in LUAD and was adjusted for purity by using TIMER database. (D) The expression correlation of PDIA3 with PD-1 in LUAD was determined by using GEPIA database. (E) The expression correlation of PDIA3 with PD-L1 in LUAD was determined by using GEPIA database. (F) The expression correlation of PDIA3 with CTLA-4 in LUAD was determined by using GEPIA database.

Article Snippet: After blocking the membranes with 5% skimmed milk at room temperature for 2 h, we incubated them overnight with rabbit anti-GAPDH polyclonal antibody (cat. No. 10494–1-AP, Proteintech, Wuhan, China) and rabbit anti-PDIA3 polyclonal antibody (cat. No. 15967–1-AP, Proteintech, Wuhan, China) at 4 ◦C.

Techniques: Expressing